Spanish version: https://goo.gl/59KHk8
French version: https://goo.gl/LR5Uis
The efficiency and potential application of a new synthetic material for vascular regeneration in arteriosclerosis is shown in porcine arteries
Spanish and British scientists, led by the University of Granada (UGR), have proven that a new polymer called 8g7 lowers the risk of thrombosis and improves the mechanical properties of vessels
Spanish and British scientists, led by the University of Granada (UGR), have proven for the first time in pig arteries that a new polymer (synthetic material) called 8g7 is efficient in the vascular regeneration of the endothelial (internal) layer in decellularized arteries. This polymer reduces the risk of thrombosis and improves the mechanical properties of vessels.
This interdisciplinary investigation, the result of collaboration between Spanish national and international institutes, has been published in the prestigious journal Scientific Report, by the Nature group. Researchers from the University of Granada, the University of Jaen, the Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), August Pi i Sunyer Biomedical Research Institute (IDIBAPS), and the Madrid-based consortium Centro de Investigación Biomédica en Red (CIBER) also working in the University of Edinburgh, Great Britain have participated in this project.
Vascular diseases or disorders affect a large amount of the population worldwide. These diseases are mainly caused by arteriosclerosis, brought about by an increase in the thickness of the internal layer, or intima, of the arteries. In many cases, the final treatment requires a vascular replacement, and alternatives such as synthetic grafts or autologous vessel implants (from the patient himself) are of limited availability.
In this study, a vascular regeneration model was developed for obtaining vascular grafts from decellularized arteries. In order to do so, the researchers used porcine carotid arteries, which were subject to an elimination process of the cells (decellularization).
Subsequently, the inside of the arteries were coated with a synthetic polymer, 8g7 (ethylmethacrylate-co-diethylaminoethylacrylate) to facilitate the growth of endothelial cells and minimize platelet attachment. This polymer was developed in the University of Edinburgh School of Chemistry by professor Mark Bradley’s group, including doctors Seshasailam Venkateswaran and Salvatore Pernagallo.
The results show that the polymer not only improves the regeneration of the endothelial layer in decellularized arteries, but improves biomechanical properties, thus representing a new strategy to facilitate re-endothelialization, or the regeneration of damaged endothelium in vascular grafts. This could have future applications in regenerative medicine for the treatment of vascular disorders.
Bibliographical Reference:
Poly (ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement.
Elena López-Ruiz, Sesha Venkateswaran, Macarena Perán, Gema Jiménez, Salvatore Pernagallo, Juan José Díaz-Mochón, Olga Tura-Ceide, Francsico Arrebola, Juan Melchor, Juan Soto, Guillermo Rus, Pedro J. Real, Maribel Díaz-Ricart, Antonio Conde-González, Mark Bradley, Juan Antonio Marchal.
Scientific Reports7(1), Article number: 407 (2017). doi: 10.1038/s41598-017-00294-6.
This article is available at: http://www.nature.com/articles/s41598-017-00294-6
Attached images:
Part of the research team, from left to right:Macarena Perán, Juan José Díaz-Mochón, Salvatore Pernagallo, Juan Antonio Marchal, Gema Jiménez, Guillermo Rus, Elena López and Juan Melchor.
Characterization of a porcine carotid arterial tissue before and after cellular elimination (decellularization), and after recellularization once treated with the poylmer 8g7.
Images taken by confocal and electron microscopy of the transmission in the internal face of the recellularized arteries after coating with the polymer 8g7.
Contact:
Juan José Díaz-Mochón
GENYO – the Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research
PTS Granada
Avenida de la Ilustración, 114
18016 GRANADA
Tel: (+34) 654 596 676
E-mail: juandiaz@ugr.es
Juan Antonio Marchal Corrales
UGR Department of Anatomy and Human Embryology
School of Medicine
Tel: (+34) 958 249 321
Centro de Investigación Biomédica (CIBM)
Tel: (+34) 958 241 000. Ext. 20080
E-mail: jmarchal@ugr.es
